The Arrest Of Vessels Has Long Proven To Be A Controversial Exercise Essay

The Arrest Of Vessels Has Long Proven To Be A Controversial Exercise In The Law Of England And Wales - Essay Example Conclusion 6. Reflective review 7. Bibliography 1. Introduction a) Synopsis Pre-judgement security of claims and post-judgement execution of a suit are of importance in maritime law of any country. In both cases, it is the maritime creditor who is at the receiving end with concerns about the fulfilment of the credit extended to the debtor, as normally, most ships are credited and it is the concern of the creditor to realize the debt amount from the debtor. The concerns of the creditor become manifold when the debtor and the property under debt from the creditor are under litigation in the purview of the concerned admiralty law of the state. This calls for a study of the relevant processes and procedures involving the application of the international maritime laws to identify and evaluate the available methods and impact of the laws on the interests of the creditor and also the other related stakeholders of the ship1. Admiralty law derived from English law and the international mariti me law have relevant sections that state the process and procedures for the arrest of vessels, the ship owner and the ship which will be studied and which will be useful to understand the process of arrests, the ways in which the pre-judgement is carried to ensure that post-judgment is enabled after the suit is accepted and the litigations of the ship owners, creditors, sister ships, and also the issue of payment to the plaintiff and any other claimant of the property of the ship owner is carried with this knowledge. The Arrest Convention of 19522 and the ratified Arrest Convention of 19993,4,5,6 along with the Supreme Court Act 1981, at sect. 20(2)7 and sect. 218(1) will be studied to understand the probable scope and litigations arising out of the arrest of vessels as per the Admiralty law while considering its jurisdiction. This knowledge is used to suggest useful recommendations that can be practically implemented within the ambit of the English jurisdiction. The following secti ons discuss the aims and objectives of the paper, followed by a literature review of the laws of Admiralty, International Maritime Law, and the procedures for arrest, conflicts arising out of the etc. The research is commissioned by The British Shipowners' Federation and the report is targeted at outlining the effectiveness of arrest procedures in the UK and the possibilities for future reforms within the scope of international maritime law. b) Aims and objectives Aims- The aims of the research are to prepare a report for the British Shipowners’ Federation by: Exploring the literature available Admiralty Law and International Maritime Law based on English Law to develop critical competence of the information available and to reflect upon it Identify and evaluate the procedures for arrest of vessels in the British Admiralty Law and any conflicts with maritime laws of other countries Provide evidence for action research and need for critical reflection to recommend improvements to the Admiralty Law Objectives- To explore the different laws existing in relation to the arrest of vessels in the UK To identify any deviations in the British Admiralty Law from those of other countries that distinguishes between the procedures for vessel arrest To understand how the deviations in the laws concerning arrest of vessel impact the creditor or the ship owner To reflect upon the impact of the applicability of laws of the different countries on vessel arrest To plan and design a reflective learning report to improve

Biotech Ethics and Advancement Essay Example for Free

Biotech Ethics and Advancement Essay Biotechnology: Of Ethics and Advancement Thousands of years ago, early man, like the rest of earths fauna and flora, was at the mercy of the elements; we relied on natures gratitude when it came to finding food and survival. As Charles Darwin would say, only the fittest could survive Mother Natures test. Eventually, however, a fully-evolved man turned the tide of the competition; we began to domesticate the once savage beasts we hunted and competed with like wolves and horses turning them into livestock and our own companions bred out of their natural ferocity, to grow our own food for our own and tart agriculture, and eventually we started to settle down into permanent communities. Man had transcended nature when the first steps of human civilization began to grow. Since then, man had crafted its own grand design for himself; the discovery of metals like Bronze and Iron, to the Pyramids of Egypt and Cities of Rome, and the rest, as they say, Is modern history. In the Modern world, we still have that said control, and with It we have now intrinsically used our knowledge to design nature herself In our Ideal Image. Technological Advancements have allowed us wield it as we pleasure. We can now edit Mother Natures work and remake It anew through our vast Interference with the body that is Science. The presence of GMOs (Genetically Modified Organisms), Dolly and all her Cloned brethren, and Real life Cyborg-Animals are Just samples of what weVe done to her. Eventually, because of this, someone Just had to say It: When will it be too much? When will we know when weVe gone too far? That Is where Ethics falls in, our man-made balance of whether or not, In the most basic sense, what we do Is right or wrong. Ethics has long been a question of how much more we can do to these once all natural things and make them artificially enhanced. When can we truly say that we, the masters have abused our power? One cannot mistake, however, that human alteration of natural things Is only a recent occurrence. Like I said, since the earliest ages of man we already tampered with nature as we sought. We turned once vlclous and wild wolves, through our process of selectively breeding the weaker ones, Into the loyal and lovable K9 friends we have today minus the genes of aggression. Pavlov strapped d own dogs to make psychological breakthroughs In condltlonlng. World War 2 scientists strapped bombs to dogs, dolphins, and even bats to assist them In fighting the enemy. Indeed, this tinkering of human hands has always been there. It now falls onto us people of today to see If things can still be set as Is. How much more can we do for our species own satisfaction? The Ethical question must always be present whenever we trod upon something as sensltlve as the condltlon of llvlng things. Animals, Ilke any other llvlng being, have xtremes in straining those rights to our needs. The uncertainty of Science itself shows that we cannot always control what happens to them come experiment time. The fear of abusing these innocent creatures is always on the minds of the very ethical/moral such as Animal Rights groups and such. Modern Genetics and Technology have sometimes crossed lines for the sake of advancement, such as taking away a creatures autonomy to turn them into natural robots. If this is truly how far Advancement has gone into sacrificing Lifes basic rules, then these righteous people really do have a reason to riot and protest.

Effect of the Moon on Planet Earth

Effect of the Moon on Planet Earth Josh Chaplin â€Å"The Earth would be a very different place without the moon. Discuss†¦Ã¢â‚¬  It’s true that without the moon, the earth would be a less romantic place. Mythical werewolves would never have been conceived, nor would we have a calendar based on the concept of the lunar phases. It has undoubtedly influenced human culture over millennia, but can the same be said for our neighbouring celestial satellite in geological terms? A seemingly insignificant lump of rock in comparison, it can be hard to comprehend that the moon has had such potentially significant impacts on shaping the earth as it appears today. But as it seems, a chain of events were set in motion from the instant the moon was formed which have not only left us here perplexed by it, but have left us here in the first place. The bulk of the moon’s responsibility for impacting upon the planet lies with its gravitation and proximity to the earth. Such a scenario allows for it to have a profound influence on the tides of our oceans, which in turn serves to slow the earth’s rotation and hurl the moon further away from us. The gravitational attraction of the moon is also the stabilising factor in a celestial battle between the large bodies of the solar system to throw the planet’s axial obliquity off-balance. The very fact that the moon is here in the first place tells of how fortunate the impact that formed it was, because were it not for that humble collision over 4.5 billion years ago, life on earth would be vastly different today at the very least (and conceivably even absent at worst). Its presence has also stimulated the application of mathematics and induced superstition in generations of humans, whilst providing total solar eclipses which are a universally rare, defining aspect of earth. The regular monthly cycle of the lunar phases has also been linked to mating sequences, hunting rituals and even the menstrual cycle, which 51% of the earth’s human population will experience for a large quantity of their lives. On top of all of this, the moon has defined the scenery of the night sky along with the stars and reflected the sun’s light to dampen its pitch black darkness since time immemorial, achieving omnipresence in a multitude of modern media. â€Å"The earth would be a very different place without the moon†. It only seems prudent to commence with the earliest chronological appearance of the moon. The most widely accepted modern theory for its formation is centred around a hypothetical protoplanet by the name of Theia. It is proposed to have been around the size of Mars, and about 10% of the mass of the earth. [1] Isotope analysis of lunar rocks bought back from the Apollo mission tells us that Theia is hypothesised to have collided with the earth at 4.527  ± 0.010 billion years before present. [1] Earth as it was back then would have been wholly transformed by this impact, altering its composition and ultimately allowing it to become the planet it is today. This collision would have also produced a considerable amount of debris, which would have subsequently accreted to form the moon. [2] This is the only feasible model which explains why the moon finds itself in orbit with the earth; physics- based computerised reconstructions show that it would not have been possible to capture a pas sing-by moon with the gravitational field of the earth, nor would it have been possible to originate from ejection of material from the molten earth due to fission by centrifugal force. [3] Assuming that this hypothesis is correct, it is obvious that earth has been extensively altered because of the moon. For one, upon impact, material from the dense iron core of Theia would likely have sunk towards the core of earth due to gravity, whilst mantle material would likely have been accreted onto the surface of the early earth. [2] This is the reason for the characteristic inner layers of the earth today. The moon would have then formed from excess material from the impact coalescing in the surrounding vicinities of the early earth. Thus, the formation of the moon both added and took away material from the early earth, heavily influencing its very composition from as early as 4.537 Ga. [1], [2] Having considered that this moon-forming impact would have been a major source of much of the terrestrial iron found on earth today, the size of our iron-nickel core would have been directly affected by it. The earth’s mantle chemically differentiated in an event called the iron catastrophe, throughout the first 500 million years of the planet’s formation. Extremely large quantities of iron succumbed to gravity and sunk to form the core. The innermost part of the earth was thus comprised of conductive elements, an iron-nickel alloy, which became able to generate electrical currents whilst rotating due to the coriolis effect in interaction with convection in the mantle [8] (which originated in the first place from heat escaping from the core). As a result, the roughly dipolar magnetosphere was conceived, giving rise to the radioactive Van Allen Belts by trapping charged protons and electrons in concentric bands surrounding the planet. [8] Figure 1 above is a scaled repre sentation of the invisible magnetosphere and Van Allen belts surrounding the earth. Only discovered in 1958, the infamously ‘deadly’ belts have been unvoiced yet fundamental in the development of life on our planet. This is due to the particles’ ability to prevent horrific ionising radiation to reach the surface of the earth and effectively fry anything which ever endeavoured to exist on the surface. The magnetosphere itself would also have prevented any charged particles of solar wind from reaching the earth’s surface and causing similar damage. Amongst other variables, the strength of the earth’s magnetic field would be directly proportional to the size of the core according to dynamo theory, [8] and therefore we have the moon-forming impact to thank for a hospitable and agreeable planet. It’s therefore fair to say that without the moon coming into fruition, the earth may never have done either. There are more obvious ongoing effects of the moon on the earth today though, than there were back in the Hadean. It is fairly common knowledge that the moon has influence on the tides of our seas and oceans. Along with the sun, it produces the twice-daily rise and fall of the seas that boggled coastal dwellers for millennia prior to Newton’s formulation of the universal law of gravitation in 1687. Naturally, the gravitational attraction between two separate entities is inversely proportional to the distance between them. [4] Thus, whilst the sun may be roughly 400 times as large as the moon, it is (coincidentally) around 400 times further away than it, and so exerts less influence over the tides. [4] The area of the earth closest to the moon at any given point will see a protuberance of its oceans, as the water is attracted to the moon’s gravitational field. [4] Simultaneously on the opposite side of the earth furthest from the moon, the crust itself succumbs to the lunar gravitation and is, in effect, marginally subsided, producing an additional oceanic bulge. [4] Figure 2 (right ) illustrates and annotates this gravitational phenomenon, by ever-so-slightly exaggerating the potential bulge of the tides! However, depending on the topography of shoreline localities and nature of continental slopes around the globe, the fluctuation can vary wildly between low and high tides. [4] In extreme cases, this can affect the livelihoods of littoral inhabitants by dictating fishing schedules or putting their homes in danger, showing how the moon really is a foremost influence on making the earth the place it is. One such scenario is the extraordinary tidal range at the Bay of Funday in Eastern Canada, which can surpass 12 metres. [4] Circumstances like this can occur upon the arrival of ‘spring’ tides (from the German verb springen, ‘to leap’, not from the name of the season) whereby the sun and moon align, causing maximum attraction in their direction and thus amplifying the height of the tides. [4] Alas, the moon is a dictatorial authority on the tides of our oceans, and presumably has been since the oceans formed around 3.8 billion years ago. Swishing and swashing the oceans for eons of geological time has not passed by without its consequences however. The moon’s gravity has created the tides on the one hand, whilst the rotation of the earth has slightly offset the location of them on the other; the actual location of the peaked tidal bulge is slightly ahead of where it would logically be, at the closest point on the earth’s surface to the moon. [7] As a result, a surprisingly large amount of mass (the tidal protuberance of the oceans) is offset slightly from the closest locality on earth to the moon at that point in time, meaning that a certain quantity of the gravitational pull is no longer directly between the earth and moon, but at a 90 ° angle to it. [7] Thus, torque is effectively created between the two planetary bodies, [7] and is often called ‘tidal friction,’ ‘tidal acceleration’ or ‘tidal braking’. This means that the presence of the moon causes our charac teristic 24 hour days to lengthen by around 2.3 milliseconds every century. [7] Taking Newton’s third law of equal and opposite reactions into account, the earth is also pushing the moon away by 3.82 ±0.007 cm per year as a result of this ‘torque’. [7] Would all of this really mean though that without the moon, the earth would be a very different place? Extrapolating back in time to 4 billion years ago tells us that the moon was some 15,000 km closer. Tidal forces would have been gargantuan, with hypothesised constant tsunami waves ravishing the planet. Perhaps, this would not only have served to shape the landscape by causing erosion, but it would have also dictated when proportions of the land surface would have been settled enough for life to flourish. In addition, the day would have been much shorter, with the year being around 400 days long due to the faster rotation of the earth. Looking ahead to the future, the earth may very well slow until it reaches t he same rotational speed as the moon, and then the exact same visage of the moon will always face the earth as in the Pluto-Charon arrangement on the outskirts of our solar system. [7] This demonstrates how that over short periods of time, the consequences of the moon’s presence on the earth are subtle, nigh negligible, but are not to be taken lightly in the (very) long run. Moving on from the tides of the planet, there is another reason in addition to the earlier-discussed Van Allen belts that the moon may well be the reason that life exists on earth as it does today. The axial tilt of the earth (also referred to as obliquity, a Milankovich cycle) is currently measured at 23.4 ° (and decreasing) between the earth’s rotational axis and the perpendicular to its orbital plane. [5] Whilst all sizeable bodies in our solar system (such as the sun and the gas giants) have an effect on this angle of tilt, the much closer proximity of the moon means that it is the most regulatory factor in this cosmic gravitational tug-of-war. [5] Figure 3 (left) shows the range between the earth’s minimum and maximum axial tilt values, for which we have the moon to thank for keeping the planet within those parameters. Without such a valuable sidekick, the tilt of a planetary body could incline wildly. In fact, there is evidence that Mars has tilted by up to 60 ° in the past, [5] presumably no thanks to the inferior gravitation of Phobos and Deimos in comparison to our moon. In a more horrific circumstance, computer models have liberated the earth of the moon’s gravitational effects and shown that it could tip by as much as 85 °, essentially interchanging the locations of the equator and the poles! [5] The would-be climatic consequences of such an event are naturally rather sketchy, but it is safe to presume that life on land would have been hard-pushed to adapt and may very well have been diminished. [5] Extrapolating from this, perhaps life on earth would be completely different, with organisms such as thermophiles at oceanic ridges, migratory birds and aquatic beings flourishing in the absence of land-dwelling mammals. It’s hard to imagine that the moon has the potential to command the diversity of the species in existence on the planet, and that it has been maintaining our climate and giving us our seasons, all due to the fact that it is steadying our axial tilt. This is the reason why the moon is such a prominent part of the ‘Rare Earth’ hypothesis, which explains how there are many different astrological criteria which must come together in order for a planet to prospectively bear life; [6] in other words, we owe our existence to the moon. Regardless of our location in the galactic habitable zone, our rocky terrain (not gaseous) and the fortune to have evolved beyond microbial life, the arrival of the moon was the ultimate (and perhaps the flukiest) stroke of luck to have ever graced the planet from a human perspective. [6] In conclusion, it’s obvious to draw from these analyses that the earth simply wouldn’t be the same without the moon, not only from a geological perspective, but from every perspective conceivable when its role in putting us here in the first place is considered. The moon is receding from our planet, and only time will tell if earth will succumb to life without it. References [1] Wieczorek, M. et al. (2006)The constitution and structure of the lunar interior Pages 322-323 [2] Canup, R.M. (2004) Simulations of a late lunar-forming impactIcarus Issue 168, Pages 433–436, 453-456 [3] Stroud, R. (2009)â€Å"The Book of the Moon† Pages24–31 [4] Grotzinger, J. Jordan, T. (2010) â€Å"Understanding Earth† Sixth Edition, Pages 540-541 [5] Dartnell, L. (2007) â€Å"Life in the Universe, a Beginners Guide† Pages 69-70 [6] Ward, P.D. Brownlee, D. (2000) â€Å"Rare Earth: Why Complex Life is Uncommon in the Universe† Pages 191, 194 200 [7] Chao, B.F. Ray, R.D. (1998) â€Å"Oceanic tidal angular momentum and Earths rotation variations† Page 403 [8] Glatzmaier, G.A. Roberts, P. H. (1995) A three-dimensional self-consistent computer simulation of a geomagnetic field reversalNature Issue377Pages 203–209 Image References Figure 1 – http://www.nasa.gov/mission_pages/sunearth/news/gallery/Magnetosphere.html Figure 2 – http://science.howstuffworks.com/moon4.htm Figure 3 – http://earthobservatory.nasa.gov/Features/Milankovitch/milankovitch_2.php 1

Planck V. Indiana :: essays research papers

Planck v. Indiana In the reviewing the case of Planck v. Indiana, many complicated issues arise. Included in those, individual rights conflicting with the public good are among the most difficult. According to Mr and Mrs. Planck's attorney, John Price, the Planck's religious beliefs prohibit them from accepting professional medicine practice, as they practice alternative medicine and home school their children. After a complaint from an older Planck daughter, who did not embrace or respect her family's lifestyle, the state was called in to investigate the health of the Planck children. In a preliminary check by the state of Indiana for eyesight, Lance Planck was found not to be in need of any service. Despite this finding, the Madison County Superior Court ordered that all of the Planck's children's eyes be examined by the state. One month after the Court ordered this, twenty armed officers with guns drawn came to the Planck's residence and commanded Mr. and Mrs. Planck to give up their children. Mr. Planck told the officers that he did not know why they were there, was pushed to the ground and had loaded rifles pointed at him. The children were then forcibly removed from their parents custody, and at no time was any identification shown by the officers. Curt, Lance Planck's younger brother, resisted this removal from his house, and was threatened by an officer that he would be "dragged out of here." After this scene, Emily, Stephen, and Curtis Planck were loaded into a van and driven to an eye doctor in Anderson, Indiana. The examining doctor, Dr. Joseph Woschitz, came to the conclusion that no treatment was needed for any of the children. How can the state justify this type of behavior? Is ripping a child unwillingly from his mother's arms in the best interest of the public good? What does society have to benefit from this? In short, this does not affect the public good per se, but does affect the Plancks and any other family that practices a religion that is not widely accepted. Following the above events, Mr. and Mrs. Planck were subsequently arrested, had their First Amendment rights violated, and had their home invaded by armed SWAT team members who fired a CS tear gas canister into their house. Simply, Mr. and Mrs. Planck and their children were targeted by the state selectively because of their religious beliefs which they manifested in home education and the practice of alternative medicine. The fundamental argument here is that the Planck's rights have been violated, and the State of Indiana has overstepped its duty of caring for the Planck's children.

Emotional (Intelligence) Quotient Essay

Aside from the person’s measure of intelligence or is Intelligence Quotient (IQ), he also has another characteristic that measures his ability to feel. It is his Emotional Intelligence, which is measured through his Emotional Intelligence Quotient (EQ). EQ is the description of one’s abilities, capacities, or skills regarding his perception and management of emotions. It is usually of his emotions, of others, and of groups of people. The concept about this emotional intelligence is a rather new field in psychology that is why its definition is changed from time to time (Research Machines plc). Way back in the 1900’s emotional intelligence were emphasized as various aspects of mental intelligence, cognitive aspects like memory and problem solving. Afterwards, even non-cognitive aspects were recognized as important factors, like the social intelligence, or the understanding and management of other people. Later in 1985, Wayne Payne explicitly discussed in his doctoral thesis the concept of emotion, wherein he tackled on developing emotional intelligence. However, the article was not published until 1990, when Peter Salovey and John D. Mayer published it (SearchCIO. com). Emotional Intelligence was then defined by Salovey and Mayer as a person’s capability of monitoring his and others feelings and emotions, analyzing it and using that set of information as a guide in his actions and thoughts. Afterwards, it has been revised to be the ability of emotion perception wherein it integrates emotion in order to facilitate or manage thoughts, understanding emotions and further regulating it, thus promoting personal growth. It is used by people in order to move around its social environment, understanding every part and every aspect of his surroundings. Emotional Intelligence encompasses a person’s ability to perceive emotions, wherein he is able to detect and decipher these feelings of emotions by looking in their faces, pictures, voices, along with their cultural background. Another is to be able to use these emotions, where he utilizes it in facilitating various activities like computing, thinking and eventually solving a problem. It is also important to understand emotions, to be able to comprehend various emotional signs and language and to be able to appreciate complicated relationships with various emotions. Finally, it would boil down to managing these various emotions, by regulating it for one’s own purpose and for others. It could be used to achieve certain goals by harnessing these emotions.

Free Essays on Phenomenal Woman

† (Shelton 20). Angelou is a very respected individual despite the fact that she was a black woman growing up when prejudice towards blacks was a major issue and women had little or no rights. In her poems, â€Å"In My Missouri,† â€Å"Equality,† and her novel I Know Why the Caged Bird Sings it is evident that Angelou has led a very rough, but outstanding life and her life experiences are truly reflected in her writings. Angelou’s parents were more fortunate than most black families. Her father, Bailey Johnson, was a navy cook, and her mother Vixian Baxter, was a nightclub performer as well as a owner of a large rooming house in San Francisco (Andrews 19). In the early 1930’s, sadly her parents were divorced and â€Å"...her father sent her and her brother, Bailey, by train, with name tags on their wrists, to live with his mother, Momma Henderson† (Andrews 19). When Angelou turned seven years old she wished to move back to her mothers house in Missouri. When Angelou was eight years old she was raped by her mother’s boyfriend. Angelou was so devastated by the rape that she refused to speak for approximately five years. Because she was so traumatized by her rape she began reading wildly which sparked her life long career (Andrews 19). Angelou says about her own rape experience, â€Å"The act of rape on a eight-year-old body is a matter of the needle giving because the camel can’t. The child gives, because the body can and the mind of the violator can’t†Ã¢â‚¬â„¢ (Magill 216). Angelou also wrote a poem about her mother’s boyfriend who raped her. The poem is titled â€Å"In my Missouri.† This particular poem expresses her feelings towards him. In my Missouri I had known a mean man ... Free Essays on Phenomenal Woman Free Essays on Phenomenal Woman Maya Angelou, born, Marguerite Johnson, was sent along with her brother to live with their grandmother in Stamps, Arkansas, when her parents were divorced. Growing up, she learned what it was to be a black girl in a world whose boundaries were set by whites: â€Å"As a child she always dreamed of waking to find her ‘nappy black hair’ metamorphosed to a long blonde bob because she felt life was better for a white girl than for a black girl† (Rigney 45). Despite the odds, her grandmother instilled pride in young Marguerite, with religion as an important element in their home. After five years of being apart from their mother, Marguerite and her brother, Bailey, were sent back to Saint-Louis to be with their mother. This move would change Angelou's life dramatically. While living in Saint Louis, she was raped by her mothers’ boyfriend. After which she confided in her brother what had happened, and soon the person was found dead. Young Marguerite believed her voice had killed him this caused her to become mute for nearly five years. Her dysfunctional childhood spent moving back and forth between her mother and grandmother caused her to struggle with maturity. She became determined to prove she was a woman and began to rush toward maturity. Because of her past, Maya Angelou went on to be an inspiration to men and women across the nation. She has the unique ability to challenge the divisions of race and class throughout her books of poetry and her autobiographies. Angelou is probably the most widely recognized figure of a poet in contemporary U.S. culture. Her work is found on television, in the movies, on the radio, in print, on the Internet, and at the 1993 presidential inauguration. Given this extraordinary career, she also serves as an exemplary figure to women undergoing many difficulties related to domestic violence and sexual abuse. She has been through what they are encountering, and she offers an important message ... Free Essays on Phenomenal Woman Strengths of a Woman Through the Looking Glass with Maya Angelou’s Poem â€Å"Phenomenal Woman†. As we go through life, we have many experiences such as reading poetry. It may not be fun all the time, but it is one of those things that, whether we can admit it or not, is a good experience for all of us. To write good poetry authors try to write what they know. Some write about experiences they have had, some write about how they were brought up, and some write about what they like or dislike. Maya Angelou uses these things in â€Å"Phenomenal Woman†. The poem not only shows her upbringing but also comes from life experiences and her own likes and dislikes. Maya Angelou, born April 4, 1928 as Marguerite Johnson in St. Louis, was raised in segregated rural Arkansas. Angelou and her brother lived with their grandmother. She married an American freedom fighter and lived in Cairo, where she was editor of The Arab Observer, the only English-language news weekly in the Middle East (Schafer 1). Maya grew up during The Great Depression and she suffered the brunt of segregation. â€Å"She was the first black and first woman street car conductor in San Francisco (during WWII).† Maya took a big part in the civil rights movement because of what she suffered while growing up. â€Å"In 1960-1961, she was asked by Martin Luther King Jr. to be the northern coordinator of the Southern Christian Leadership Conference† (Donegal 1). These are a few of the things that she experienced in life that help her to write meaningful poetry such as â€Å"Phenomenal Woman†. She got strength from growing up during a racially segregated time and working with strong individuals. We can see this strength through what she has accomplished thus far in her life. Some of the poetry Angelou has written is â€Å"Still I Rise†, â€Å"Men†, â€Å"Remembrance†, â€Å"A Conceit†, â€Å"Touched by an Angel†, â€Å"Refusal†, and â€Å"The Lesson† (Al... Free Essays on Phenomenal Woman Phenomenal Woman Maya Angelou was born in St. Louis, MO, on April 4, 1928, and is still alive today. As a child, she faced many hardships and traumas. When Angelou writes she has the â€Å"phenomenal power of rhythm of words† (Shelton 20). Angelou is a very respected individual despite the fact that she was a black woman growing up when prejudice towards blacks was a major issue and women had little or no rights. In her poems, â€Å"In My Missouri,† â€Å"Equality,† and her novel I Know Why the Caged Bird Sings it is evident that Angelou has led a very rough, but outstanding life and her life experiences are truly reflected in her writings. Angelou’s parents were more fortunate than most black families. Her father, Bailey Johnson, was a navy cook, and her mother Vixian Baxter, was a nightclub performer as well as a owner of a large rooming house in San Francisco (Andrews 19). In the early 1930’s, sadly her parents were divorced and â€Å"...her father sent her and her brother, Bailey, by train, with name tags on their wrists, to live with his mother, Momma Henderson† (Andrews 19). When Angelou turned seven years old she wished to move back to her mothers house in Missouri. When Angelou was eight years old she was raped by her mother’s boyfriend. Angelou was so devastated by the rape that she refused to speak for approximately five years. Because she was so traumatized by her rape she began reading wildly which sparked her life long career (Andrews 19). Angelou says about her own rape experience, â€Å"The act of rape on a eight-year-old body is a matter of the needle giving because the camel can’t. The child gives, because the body can and the mind of the violator can’t†Ã¢â‚¬â„¢ (Magill 216). Angelou also wrote a poem about her mother’s boyfriend who raped her. The poem is titled â€Å"In my Missouri.† This particular poem expresses her feelings towards him. In my Missouri I had known a mean man ...

Analytical Characterization of Fatty Acids Essay Example

Analytical Characterization of Fatty Acids Essay Example Analytical Characterization of Fatty Acids Essay Analytical Characterization of Fatty Acids Essay 2 Besides its familiarity for toxicity and poisoning,3 it has uses for a number of diseases like asthma, muscle spasm, whooping cough, skin ulcer, hemorrhoids, anti-rheumatic etc. Its oil based preparation is used for healing of all types of wounds in Ayurveda and Siddha practice of medicine since long ago. 4 Datura species produces a number of small seeds encapsulated in an apple like fruit capsule; hence the name â€Å"thornapple† is based on this fact. The most common and medicinally important tropane alkaloids such as hyoscyamine, atropine nd scopolamine have been isolated from Datura species. Clarification of the dual effect of this plant requires extensive research for the exploration of chemistry and pharmacology of the plant under investigation. The present work is a part of these investigations focusing on the determination of fatty acids in oil extracted from Datura seeds. Due to biological importance5,6 fatty acids have gained importance in food nutrition evaluation 7-10 and in the diagnosis of certain diseases and pharmacology. 1 Fatty acids with unsaturation, either monounsaturated or polyunsaturated, have been used in lowering the risks of heart disease, against inflammation and in enhancing the immunity or immune system. 12-17 A number of analytical techniques have been applied for the determination of fatty acids. These include: enzymatic, spectrophotometric, HPLC18-20 and gas chromatography (GC). 21-23 GC-MS is the method of choice for the analysis of fatty acids due to various reasons like speed, resolutions and sensitivity. 4,25 From the literature it appears that most of the research work performed is the determination of alkaloids which are main constituents of this genus. No remarkable work has been carried out for the determination of fatty acids in Datura alba seed oil which is needed in order to explore its pharmacological importance. 2. RESULTS AND DISCUSSION Table 1 summarizes the results obtained from the GCMS analysis showing the relative concentration of individual esterified fatty acids based on the external standard method and the standard deviation values among the three results in each case. Analyses were performed three times and the values of area and concentration in Table 1 are the average of three measurement results. Quantification of FAMEs was performed using three points calibration curve * Corresponding author. Tel: 0092-91-9216240-43; Fax: 0043-512-5072767; E-mail: [emailprotected] com Fatty Acids in Datura alba Seed Oil by GC-MS J. Chin. Chem. Soc. , Vol. 58, No. 2, 2011 237 Table 1. Quantification results of fatty acid methyl esters # 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 Name C6:0; Hexanoic acid, methyl ester C8:0; Caprylic acid, methyl ester C10:0; Capric acid, methyl ester C12:0; Lauric acid, methyl ester C14:0; Myristic acid, methyl ester C15:0; Pentadecanoic acid, methyl ester C16:0; Palmitic acid, methyl ester C16:1c; Palmitoleic acid, methyl ester C17:0; Margaric acid, methyl ester C18:0; Stearic acid, methyl ester C18:1c; Oleic acid, methyl ester C18:1n8T; Octadecanoic acid, methyl ester C18:2c; Linoleic acid, methyl ester C20:0; Arachidic acid, methyl ester C20:2; Eicosadienoic acid, methyl ester Retention time (min. ) 3. 060 4. 957 6. 796 8. 552 11. 000 12. 670 14. 692 15. 213 16. 985 19. 704 20. 266 20. 433 21. 912 27. 306 29. 756 AreaO 19654 11442 3454 2928 39973 2407 2674581 8191 13220 427823 816146 86541 1554375 30982 22200 Conc. (%)O 0. 16 0. 11 0. 02 0. 1 0. 18 0. 01 6. 59 0. 21 0. 09 1. 35 5. 41 0. 92 16. 22 0. 22 0. 65 Std. Dev. * 0. 002 0. 003 0 . 002 0. 003 0. 010 0. 003 0. 020 0. 002 0. 003 0. 010 0. 003 0. 004 0. 002 0. 004 0. 005 * Standard deviation values for the three measurement results; O Average of three measurement results. with R2 value less than 0. 99 (R2 0. 99) in each case. Fig. 1 shows the GC-MS chromatogram obtained from fatty acid standard mixture of 37 components while Fig. 2 is the GCMS chromatogram of Datura alba seed oil with properly labeled signals of analytes detected. Both the saturated and unsaturated fatty acids were found in the sample under investigations. Linoleic acid was found in highest concentration which is necessary for the maintenance of growth. It has been shown to be a potent inhibitor of cyclooxiginase-2 (COX-2) catalyzed prostaglandin biosynthesis. 29,30 Among the other fatty acids with concentrations more than 1% are: Palmitic acid (6. 59%), Oleic acid (5. 41%) and Stearic acid (1. 35%) were found. Amount of rest of the fatty acids were less than 1% (Table 1). From the results it is clear that Datura alba seeds, besides its toxicity, can also be used in various pharmaceutical products as it contains different bioactive compounds like fatty acids. The method applied is a reliable method of analyzing imultaneously many fatty acid components in a single run. 3. EXPERIMENTAL 3. 1. Chemicals and reagents Boron triflouride solution in methanol (10%) was purchased from Fluka Chemie (Buchs, Switzerland). Sodium hydroxide solution (methanolic; 0. 5 N) and sodium chloride (analytical grade) were obtained from Merck (Darmstadt, Germa ny) while methanol (HPLC grade), n-hexane (HPLC grade) were from Fischer Scientific (Leicestershire, UK). Helium gas (99. 9999%) from Pak gas (United Arab Emirates) was procured. Tridecanoic acid methyl ester and Fatty acid methyl esters (FAMEs) 37 components standard mix were obtained from AccuStandard (Newhaven, Connecticut USA). These 37 components are: methyl ester of hexanoic acid, caprylic acid, capric acid, undecanoic acid, lauric acid, tridecanoic acid, myristic acid, myristoleic acid, pentadecanoic acid, pentdecenoic acid, palmitic acid, palmitoleic acid, margaric acid, heptadecenoic acid, stearic acid, oleic acid, elaidic acid, octadecenoic acid, linoleic acid, octadecadienoic acid, g-linolenic acid, linolenic acid, arachidic acid, eicosenoic acid, eicosadienoic acid, 8,11,14-eicosatrienoic acid, heneicosanoic acid, arachidonic acid, eicosatrienoic acid, eicosapentaenoic acid, behenic acid, eruccic acid, docosadienoic acid (C22:2), tricosanoic acid, tetracosanoic acid, docosahexaenoic acid and tetracosenoic acid. Deionized water was used through out the experimental work. 3. 2. Preparation of standard Internal standard was prepared by dissolving 13. 7 mg of tridecanoic acid methyl ester in 1 mL hexane. External standard was prepared by diluting 10 mg of 37 component FAMEs mix standard to 10 mL with di chloromethane. From this solution further working standard solutions were prepared. 238 J. Chin. Chem. Soc. , Vol. 58, No. 2, 2011 Qureshi et al. Fig. 1. GC-MS chromatogram of 37 components standard. Chromatographic conditions: inj. vol. : 1  µL, carrier gas: Helium, column: TRB-FFAP capillary column (length; 30 m, i. d. ; 0. 35 mm, thickness; 0. 50  µm, treated with polyethylene glycol), MS scanning: 85-380 m/z. 3. 3. Extraction of oil and preparation of FAMEs About 100 g powdered seed material was extracted with 250 mL n-hexane26 for six hours through soxhlet extraction apparatus. The extract was concentrated by recovering the solvent using rotary evaporator. Fatty acids are polar compounds and are not volatile. For gas chromatographic analysis it is necessary that the sample to be analyzed must be volatile. In order to make fatty acids present in the oil volatile, derivatizaion is performed prior to GC-MS analysis. Methylation is the most general method of converting non-vola tile fatty acids into volatile fatty acids methyl esters (FAMEs). 7 Methylation of fatty acids was performed with BF3-methanol as derivatizing reagent, which is the most accepted procedure for converting fatty acids into FAMEs. 23 Derivatization was performed according to the AOAC standard reference method. 28 To a known amount of sample (equivalent to 25 mg fat) was added 0. 1 mL internal standard (1. 37 mg) and 1. 5 mL of sodium hydroxide solution in methanol (0. 5 N), sealed and heated in boiling water bath for 5 minutes. The hydrolyzed sample was cooled and added 2. 5 mL of boron triflouride solution in methanol (10%). The solution was then sealed and heated in boiling water bath for 30 minutes and cooled. To the esterified solution was added 5 mL saturated sodium chloride solution and extracted twice with 1 mL hexane. The hexane extract was filtered through 0. 45 mm membrane filter and injected 1 ml to GCMS using auto injector system. 3. 4. Chromatographic separation of FAMEs A gas chromatograph from Shimadzu hyphenated to a mass spectrometer QP 2010 plus (Tokyo, Japan) equipped with an auto-sampler (AOC-20S) and auto-injector (AOC20i) was used. Helium was used as carrier gas. All chromatographic separations were performed on a capillary column (TRB-FFAP; Technokroma) having specifications: length; 30 m, i. d. ; 0. 35 mm, thickness; 0. 250  µm, treated Fatty Acids in Datura alba Seed Oil by GC-MS J. Chin. Chem. Soc. , Vol. 58, No. 2, 2011 239 Fig. 2. GC-MS chromatogram of Datura alba seed oil. Chromatographic conditions: as in Fig. 1. with polyethylene glycol. Other GC-MS conditions are: ion source temperature (EI); 250  °C, interface temperature; 240  °C, pressure; 100 KPa, solvent cut time; 1. 8 min. 1  µL of sample and standard were injected into the GC column. Injector was operated in a split mode with a split ratio 1:50. Injection temperature was 240  °C. The column temperature program started at 50  °C for 1 min and changed to 150  °C at the rate of 15  °C/min. The temperature was raised to 175  °C at the rate of 2. 5  °C/min and hold for 5 minutes. Then the temperature was increased to 220  °C at the rate of 2. 5  °C/min and kept constant for 3 minutes. Total elution time was 43 minutes. MS scanning was performed from m/z 85 to m/z 380. GC-MS solutions software provided by the supplier was used to control the system and to acquire the data. Identification of the compounds was carried out by comparing the mass spectra obtained with those of standard mass spectra from the NIST library (NIST 05). REFERENCES 1. Nadkarni, K. M. Dr. KM Nadkarni’s Indian Materia Medica; Popular Prakashan: Bombay, 1994. 2. Kuganathan, N. ; Saminathan, S. ; Muttukrishna, S. Internet J. Toxicol. 2008, 5(2). 3. Steenkamp, P. A. ; Harding, N. M. ; Heerden, F. R. v. ; Wyk, B. E. v. Forensic Sci. Int. 2004, 145, 31-39. 4. Priya, K. S. Gnanamani, A. ; Radhakrishnan, N. ; Babu, M. Journal of Ethnopharmacology 2002, 83, 193-199. 5. Wallace, F. A. ; Neely, S. J. ; Miles, E. A. ; Calder, P. C. Immunol. Cell. Biol. 2000, 78, 40-48. 6. Cherif, S. ; Frikha, F. ; Gargouri, Y. ; Miled, N. Food Chem. 2008, 111, 930-933. 7. Tomaino, R. M. ; Parker, J. D. ; Larick, D. K. J. Agric. Food Chem. 2001, 49, 3993-3998. 8. Skonberg, D. I. ; Perkins, B. L. Food Chem. 2002, 77, 401-404. 9. Martin, C. A. ; Carapelli, R. ; Visantainer, J. V. ; Matsushita, M. ; de Souza, N. E. Food Chem. 2005, 93, 445-448. 10. Philip, C. C. Prostaglandins, Leukot. Essent. Fatty Acids 2008, 79, 101-108. 11. Stoddart, L. A. Smith, N. J. ; Milligan, G. Pharmacol. Rev. 2008, 60, 405-417. 12. Calder, P. Lipids 1999, 34, S137-S140. 240 J. Chin. Chem. Soc. , Vol. 58, No. 2, 2011 Qureshi et al. 13. Hamberg, M. ; Hamberg, G. Phytochemistry 1996, 42, 729-732. 14. Hargrove, R. L. ; Etherton, T. D. ; Pearson, T. A. ; Harrison, E. H. ; Kris-Etherton, P. M. J. Nutr. 2001, 131, 1758-1763. 15. Yaqoob, P. Eur. J. Clin. Nutr. 2002, 56, 9. 16. Villa, B. ; Calabresi, L. ; Chiesa, G. ; Rise, P. ; Galli, C. ; Sirtori, C. R. Pharmacol. Res. 2002, 45, 475-478. 17. Siscovick, D. S. ; Raghunathan, T. E. ; King, I. ; Weinmann, S. ; Wicklund, K. G. ; Albright, J. ; Bovbjerg, V. ; Arbogast, P. Smith, H. ; Kushi, L. H. ; Cobb, L. A. ; Copass, M. K. ; Psaty, B. M. ; Lemaitre, R. ; Retzlaff, B. ; Childs, M. ; Knopp, R. H. JAMA 1995, 274, 1363-1367. 18. Bailey, A. L. ; Southon, S. Anal. Chem. 1998, 70, 415-419. 19. Zhao, J. ; Li, S. P. ; Yang, F. Q. ; Li, P. ; Wang, Y. T. J. Chromatogr. , A 2006, 1108, 188-194. 20. Romanowicz, L. ; Galewska, Z. ; Gogiel, T. ; Jaworski, S. ; Sobolewski, K. J. Biochem. Biophys. Methods 2008, 70, 973-977. 21. Yue, X. -F. ; Zhang, Y. -N. ; Zhang, J. ; Zhang, Z. -Q. Anal. Methods 2010, 2, 668-672. 22. Rosenfeld, J. M. Anal. Chim. Acta 2002, 465, 93-100. 23. Shantha, N. C. ; Napolitano, G. E. J. Chromatogr. A 1992, 624, 37-51. 24. Destaillats, F. ; Cruz-Hernandez, C. J. Chromatogr. , A 2007, 1169, 175-178. 25. Yi, L. ; He, J. ; Liang, Y. ; Yuan, D. ; Gao, H. ; Zhou, H. Chem. Phys. Lipids 2007, 150, 204-216. 26. Anwar, F. ; Bhanger, M. I. ; Nasir, M. K. A. ; Ismail, S. J. Agric. Food Chem. 2002, 50, 4210-4214. 27. Dron, J. ; Linke, R. ; Rosenberg, E. ; Schrei ner, M. J. Chromatogr. , A 2004, 1047, 111-116. 28. AOAC 991. 39, 17th ed. ; Chapet 41, p 26, 2000. 29. Ringbom, T. ; Huss, U. ; Stenholm, A. ; Flock, S. ; Skattebol, L. ; Perera, P. ; Bohlin, L. J. Nat. Prod. 2001, 64, 745-749. 30. Badoni, R. ; Semwal, D. K. ; Rawat, U. J. Sci. Res. 2010, 2, 397-402.